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贵州传统酸菜中优势乳酸菌的分离鉴定及发酵特性分析
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徐碟(2000-),女,硕士研究生,研究方向:食品加工与安全,E-mail:2184508812@qq.com 通讯作者:林茂(1979-),女,博士,研究员,研究方向:农产品加工与品质调控,E-mail:linmao520132@163.com

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黔现代所发展基金[2024]4号


Isolation, Identification, and Fermentation Characteristics of Dominant Lactic Acid Bacteria from Guizhou Traditional Sauerkraut
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    摘要:

    为筛选适用于酸菜发酵的优良菌株,本研究从贵州传统酸菜中分离纯化出19株乳酸菌,首先根据产酸能力和生长状况进行初筛,随后基于亚硝酸盐降解能力、耐酸性、产粘性及产硫化氢性能进行复筛。结果表明,菌株RS11综合表现最优:其亚硝酸盐降解率高达98.82%;对pH值3.50的环境耐受性最佳,且不产粘、不产硫化氢。安全性评价显示,RS11无溶血活性;耐受链霉素与卡那霉素;对四环素、万古霉素和红霉素中度敏感;对氯霉素、氨苄青霉素及庆大霉素敏感。经16S rDNA鉴定为植物乳植杆菌(Lactiplantibacillus plantarum)。该菌株为同型乳酸发酵,且能够利用所有测试糖类,生长和产酸速度快:2 h进入对数生长期,12 h达到稳定期,并在12 h内将pH值降至3.98。接种发酵结果表明,与传统自然发酵相比,使用RS11可将发酵周期从7 d缩短至2 d,且发酵过程中亚硝酸盐峰值含量仅为0.62 mg•kg-1。综上,从传统酸菜发酵体系中筛选的本土植物乳植杆菌RS11兼具优异的发酵特性和安全调控功能,是适用于酸菜生产的优良菌株。

    Abstract:

    To screen for excellent strains for sauerkraut fermentation, 19 lactic acid bacteria were isolated and purified from traditional sauerkraut in Guizhou. Preliminary screening was carried out based on acid production capacity and growth status, followed by secondary screening based on the nitrite degradation capacity, acid tolerance, viscosity production, and hydrogen sulfide production properties. Strain RS11 exhibited the best overall performance, with a nitrite degradation rate of 98.8%, optimal tolerance at pH 3.50, and absence of viscosity or hydrogen sulfide production. Safety evaluation showed that RS11 had no hemolytic activity, showed resistance to streptomycin and kanamycin, showed moderate sensitivity to tetracycline, vancomycin, and erythromycin, and was sensitive to chloramphenicol, ampicillin, and gentamicin. RS11 was identified as Lactiplantibacillus plantarum through 16S rDNA sequencing. This strain underwent homolactic fermentation and was able to utilize all tested carbohydrates, exhibiting rapid growth and acid production; it entered the logarithmic growth phase within 2 h, reached the stationary phase by 12 h, and reduced the pH to 3.98 within 12 h. The inoculation and fermentation results showed that compared with traditional natural fermentation, the application of RS11 shortened the fermentation cycle from 7 d to 2 d, and the peak nitrite content during the fermentation process was only 0.62 mg•kg-1. In conclusion, local L. plantarum RS11 screened from the traditional sauerkraut fermentation systems offers excellent fermentation characteristics and safety and hence is suitable for sauerkraut production.

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徐碟,汤鹏宇,孟繁博,林茂.贵州传统酸菜中优势乳酸菌的分离鉴定及发酵特性分析[J].现代食品科技,2026,42(9):111-118.

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  • 收稿日期:2025-06-20
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  • 在线发布日期: 2026-10-10
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