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传统虾酱油中组胺降解菌的筛选鉴定及其降解效能分析
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史玉雪(2000-),女,硕士研究生,研究方向:微生物应用与发酵工程,E-mail:shiyuxue1107@163.com 通讯作者:解万翠(1969-),女,博士,教授,研究方向:海洋生物与健康食品,E-mail:xiewancui2@163.com

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国家自然科学基金资助项目(32472483;32072348);山东省科技型中小企业创新能力提升工程项目(2023TSGC0389;2023TSGC0766)


Screening, Identification, and Degradation Efficiency Analysis of Histaminedegrading Bacteria in Traditional Shrimp Sauce
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    摘要:

    为绿色控制传统发酵虾酱油中的组胺污染问题,本研究以胶东半岛传统虾酱油为原料,通过质量分数为5%的NaCl的MRS培养基分离耐盐菌株50株;通过溴甲酚紫生物胺显色培养基进行初筛,获得10株不产生生物胺的菌株;进一步结合高效液相色谱(High Performance Liquid Chromatography, HPLC)对菌株组胺降解能力进行定量分析,最终筛选得到5株具有组胺降解能力的菌株。经生理生化试验及16S rDNA分子鉴定为白色芽孢杆菌(Bacillus albus A-1)、蜡样芽孢杆菌(Bacillus cereus A-2、C-3)、枯草芽孢杆菌(Bacillus subtilis A-3)及解淀粉芽孢杆菌(Bacillus amyloliquefaciens A-4)。其中枯草芽孢杆菌A-3表现出最优的组胺降解能力(体外降解率26.67%),具备较强的环境适应力。安全性评估表明其无溶血活性,对氨苄西林等9种抗生素完全敏感,并对大肠杆菌产生抑菌圈(12.75 mm)。进一步将其应用于南极磷虾酱油快速发酵,结果显示组胺含量降至12.36 μg•mL-1(降解率38.35%),并通过定量描述分析法(Quantitative Descriptive Analysis, QDA)和电子鼻技术证实其显著提升产品感官品质。该菌株兼具高效降解能力、环境适应性及生物安全性,为虾酱油安全生产提供了可靠的微生物资源与技术支撑。

    Abstract:

    To achieve the green control of histamine contamination in traditionally fermented shrimp sauce, salt-tolerant strains (50 isolates) were isolated from traditional shrimp sauce from the Jiaodong Peninsula using MRS medium containing 5% NaCl. Ten non-biogenic amine-producing strains were preliminarily screened via bromocresol purple chromogenic medium. Five histamine-degrading strains were subsequently obtained by quantifying histamine degradation rates using high-performance liquid chromatography. Physiological and biochemical tests combined with 16S rDNA molecular analyses identified these strains as Bacillus albus A-1, Bacillus cereus A-2, Bacillus cereus C-3, Bacillus subtilis A-3, and Bacillus amyloliquefaciens A-4. Among them, B. subtilis A-3 exhibited the optimal degradation capacity (26.67% in vitro degradation rate) and robust environmental adaptability. Safety assessments confirmed its non-hemolytic activity, complete susceptibility to nine antibiotics (including ampicillin), and antibacterial activity against Escherichia coli (inhibition zone diameter: 12.75 mm). When applied in the rapid fermentation of Antarctic krill sauce, this strain significantly reduced the histamine content to 12.36 μg•mL-1 (38.35% degradation rate). The sensory qualities were enhanced, as verified through a quantitative descriptive analysis (QDA) and electronic nose analysis. B. subtilis A-3, which integrates a high-efficiency degradation capability, environmental adaptability, and biosafety, provides a reliable microbial resource and technical foundation for the safe production of shrimp sauce.

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史玉雪,李昊恩,纪凌枫,孙飞,张桂林,解万翠.传统虾酱油中组胺降解菌的筛选鉴定及其降解效能分析[J].现代食品科技,2026,42(9):62-71.

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  • 收稿日期:2025-06-27
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  • 在线发布日期: 2026-10-10
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