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基于内滤效应的水凝胶基比率型荧光探针构建及食品安全监测研究进展
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1.桂林理工大学 化学与生物工程学院;2.桂林理工大学 化学与生物工程学院 电磁化学功能物质广西区重点实验室

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Progress in the Development of Hydrogel-Based Ratio Fluorescence Probes Based on the Internal Filtration Effect and Their Application in Food Safety Monitoring
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    摘要:

    食品安全关乎公众健康与社会稳定,因此建立快速、灵敏、抗干扰的现场检测技术是当前分析化学与食品科学交叉领域的核心需求。基于荧光内滤效应(IFE)的传感策略,凭借无需对荧光供体进行复杂共价修饰、信号响应可直接转化为裸眼可辨的颜色变化的独特优势,在快速检测领域备受关注。将内滤型比率荧光探针与水凝胶耦合,构建新一代食品污染物现场快速检测平台,更是近年该方向的研究热点。本文系统阐述了IFE的传感作用机理,梳理了水凝胶基质,与比率荧光体系的构建核心策略,归纳了近年该技术在农药残留、重金属离子、生物毒素等食品污染物检测中的代表性应用案例与实际检测性能。最后针对当前仍存在的复杂食品基质干扰、凝胶长期储存稳定性不足等现实瓶颈,对未来技术优化方向进行了展望,可为相关新型探针设计与食品现场检测产品开发提供实用参考。

    Abstract:

    Food safety is critical to public health and social stability; therefore, the development of rapid, sensitive, and anti-interference on-site detection technologies has become a core demand at the intersection of analytical chemistry and food science. Fluorescence sensing strategies based on the inner filter effect (IFE) have attracted considerable attention in the field of rapid detection, owing to their distinctive advantages, including the elimination of complex covalent modifications of the fluorescent donor and the direct conversion of signal responses into visually identifiable color changes. The integration of IFE-based ratiometric fluorescent probes with hydrogels to construct next-generation platforms for the on-site rapid detection of food contaminants has emerged as a prominent research hotspot in recent years. This review systematically elucidates the sensing mechanisms of the IFE, outlines the core strategies for constructing hydrogel matrices and ratiometric fluorescence systems, and summarizes representative application cases and practical detection performance of this technology in the detection of food contaminants such as pesticide residues, heavy metal ions, and mycotoxins. Finally, in view of the current practical bottlenecks, including interference from complex food matrices and insufficient long-term storage stability of hydrogels, future directions for technological optimization are prospected. This review is expected to provide practical references for the design of novel probes and the development of on-site food testing products.

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  • 收稿日期:2026-07-17
  • 最后修改日期:2026-11-03
  • 录用日期:2026-11-03
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