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不同贮藏条件对浓缩桃汁非酶褐变的动态变化分析
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张小希(1998-),女,硕士,研究实习员,研究方向:果蔬加工,E-mail:caslu_98@163.com 通讯作者:马寅斐(1986-),男,硕士,副研究员,研究方向:果品加工技术,E-mail:149423305@qq.com;共同通讯作者:初乐(1987-),女,硕士,研究员,研究方向:果蔬精深加工技术,E-mail:785536270@qq.com

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山东省重点研发计划项目(科技型中小企业创新能力提升工程)(2024TSGC0409);山东省重点研发计划(乡村振兴科技创新提振行动计划)(2023TZXD064)


Analysis of Dynamic Changes in Non-enzymatic Browning of Concentrated Peach Juice under Different Storage Conditions
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    摘要:

    为深入研究贮藏期浓缩桃汁非酶褐变的变化规律,该研究利用紫外可见分光光度计、色差仪、高效液相色谱仪和液质联用仪分别进行不同贮藏温度下影响浓缩桃汁外观和非酶褐变的相关指标进行分析。随着温度升高,浓缩桃汁OD420 nm值、ΔE逐渐升高。在4、25和37 ℃下贮藏期间,随着贮藏时间的增加,抗坏血酸、还原糖、氨基酸和氨基酸态氮作为产生褐变的主要前提物质含量明显降低;5-羟甲基糠醛(5-Hydroxymethylfurfural, 5-HMF)含量与其相反。除此之外,相关性分析表明ΔE与还原糖、a*、b*、褐变度和5-HMF之间具有正相关性;而L*与总酚、4种氨基酸、氨基酸态氮和抗坏血酸之间具有正相关性;随着贮藏温度的提高,浓缩桃汁的非酶褐变指标聚类之间差距越大。因此,该研究发现抑制5-HMF生成可以明显改善浓缩桃汁的褐变程度,并且适量提高总酚、氨基酸和抗坏血酸的含量可以提高浓缩桃汁的光泽。该研究结果为浓缩桃汁的贮藏提供了一定科学依据,也有利于对市场果汁保存提供理性引导。

    Abstract:

    To explore in-depth the alterations in the non-enzymatic browning of concentrated peach juice during storage, a range of analytical techniques, including UV-visible spectrophotometry, colorimetry, high-performance liquid chromatography, and liquid chromatography-mass spectrometry, were employed to assess indicators relevant to the visual characteristics and nonenzymatic browning of the juice at different storage temperatures. The results showed that as the storage temperature increased, both the optical density at 420 nm (OD420 nm) and color difference (ΔE) of the concentrated peach juice increased gradually. During storage at 4, 25, and 37 ℃ , significant reductions in the levels of ascorbic acid, reducing sugars, amino acids, and amino acid nitrogenkey precursors to browning-were observed over time. Conversely, the concentration of 5-hydroxymethylfurfural (5-HMF) increased. Correlation analysis further demonstrated that ΔE was positively correlated with reducing sugars, the color parameters a* and b*, browning degree, and 5-HMF, whereas the lightness parameter (L*) was positively correlated with total phenolic content, four types of amino acids, amino acid nitrogen, and ascorbic acid. Notably, as the storage temperature increased, the disparities in the clusters of non-enzymatic browning indicators for concentrated peach juice became increasingly pronounced. Therefore, it was concluded that inhibiting the formation of 5-HMF could significantly reduce the browning degree of concentrated peach juice. At the same time, an appropriate increase in the levels of total phenols, amino acids, and ascorbic acid could improve the glossiness of the juice. The results of this study can serve as a scientific foundation for the storage of concentrated peach juice and offer practical recommendations for the preservation of commercially available fruit juices.

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张小希,李根,孔天宇,师恩娟,赵岩,赵晓丹,吕绪强,马寅斐,初乐.不同贮藏条件对浓缩桃汁非酶褐变的动态变化分析[J].现代食品科技,2026,42(7):122-131.

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  • 收稿日期:2025-04-16
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  • 在线发布日期: 2026-09-02
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