To enhance the bioavailability of ginsenoside Rg3, Rg3-PEG-PLGA nanomicelles were prepared by thinfilm hydration using polyethylene glycol-polylactic-co-glycolic acid copolymer (PEG-PLGA) as a carrier. The properties of the nanomicelles were evaluated using parameters such as particle size, encapsulation efficiency, and drug-loading capacity. This study optimized the preparation process of Rg3 nanomicelles using single-factor experiments and response surface methodology, followed by an assessment of the quality of the optimized product. The results showed that after process optimization, the drugloading capacity of Rg3 nanomicelles was 2.62% and the encapsulation efficiency was 90.99%. Additionally, the cumulative in vitro Rg3-PEG-PLGA nanomicelle release rate reached 93.60% within 72 h. The critical micelle concentration (CMC), average particle size, polydispersity index (PDI), and zeta potential were 3.67 μg/mL, 46.52 nm, 0.09, and -24.37 mV, respectively. The lyophilized powder was white powder and the reconstituted solution was milky white liquid with 52.27 nm average particle size, 0.408 PDI, and -22.44 mV zeta potential. The viscosity was approximately 0.89 mPa•s (cP). In vitro release results showed that Rg3 nanomicelles exhibited good sustained-release properties. The optimized preparation process was stable and feasible, and the resulting nanomicelles possessed uniform particle size distribution, high encapsulation efficiency, high drug-loading capacity, and sustained-release properties. The study demonstrated that this preparation method is simple and safe, laying the foundation for its future applications in the fields of medicine, food, and others. The Rg3-PEG-PLGA nanomicelle product has significant practical significance.