Guizhou Dalbergia odorifera honey, a new monofloral nectar, has high nutritional value. Metabolite analyses of four honeys from Guizhou (D. odorifera honey, Galla Chinensis honey, blueberry honey, and loquat honey) and manuka honey from New Zealand were carried out using ultra performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry (UPLC-QTOF-MS) metabolomics, and 1,788 metabolites were detected in positive and negative ion modes. Principal component analysis (PCA) showed similar metabolite compositions between D. odorifera and manuka honey, with significant differences between them and the remaining honeys. Differential metabolites between D. odorifera honey and the other four types of honey were further screened and identified (VIP>1, P<0.05). Results showed 58, 66, 60, and 67 differential metabolites between D. odorifera honey and G. Chinensis honey, D. odorifera honey and blueberry honey, D. odorifera honey and loquat honey, and D. odorifera honey and manuka honey, respectively. KEGG enrichment analysis of the differential metabolites yielded consistent performance for the first five major metabolic pathways: protein digestion and absorption, carbohydrate metabolism, aminoacyl-tRNA biosynthesis, ABC transport, and mineral absorption. In addition, the fructose, rhamnose, mannose, aspartic acid, apigenin, luteolin, ellagic acid, and resveratrol content in D. odorifera honey is high, which can be used as potential markers to differentiate D. odorifera honey from other honeys. The results of this study reveal the differences in metabolites of different honey varieties at the overall level, and provide a direction for the identification and quality evaluation of D. odorifera honey.