In this study, a deer skin gelatin peptide-Fe (DSGP-Fe) chelate was prepared using deer skin gelatin as the raw material, which was subjected to proteolytic hydrolysis and ferrous chloride (FeCl2•4H2O) treatment, and the preliminary pharmacological study was carried out on its blood-supplementing effect. The structure was analyzed using Fourier infrared spectroscopy and scanning electron microscopy; an iron-deficiency anemia model was established by a combination of feeding an iron-deficient diet and bloodletting. After the model was successfully established, the corresponding medication was given until the Hb value was higher than 110 g/L. At the end of the experiment, theorgan indexes, iron metabolismrelated serum factor indexes, liver injury-related indexes, and oxidative stress indexes of the mice were measured. Characterization results show that DSGP-Fe had a loose bulk structure, in which Fe2+ mainlybound to the chelating active groups such as amino and carboxyl groups in deer skin gelatin peptide (DSGP) to form DSGP-Fe. Ferrous succinate tablets, DSGP and DSGP-Fe increased the IDA group’s body weight (42.3, 35.7, 41.8 g), splenic index (3.58, 3.91, 3.63 mg/g) and hepatic index (2.31%, 2.02%, 2.28%), SI (32.52, 27.35, 35.47 mg/L), SF (34.34, 32.21, 36.34 ng/L) and TSAT (7.49%, 7.25%, 7.32%) levels, decreased their TIBC (4.34, 3.77, 4.84 mg/L) levels, raised their GSH (13.34, 11.34, 14.65 μmol/g prot) and SOD (209.35, 193.52, 216.58 U/mg prot) activities, and reduced ALT (27.34, 20.77, 20.34 U/L), AST (61.34, 50.21, 50.34 U/L), and MDA (1.54, 1.67, 1.52 nmol/mg prot) contents. In conclusion, DSGP-Fe exerted significant effects on iron supplementation, regulation of iron metabolism, and enhancement of anti-oxidative stress capacity in young mice with iron-deficiency anemia. Therefore, DSGP-Fe can be used as a new potential source of iron, and provides a new idea for the development of iron fortifiers from deer skin gelatin.