Abstract:Mycotoxin contamination in oilseeds is of particular concern because of their potential adverse effects, hence reliable analytical methods are required for detection and quantification. This study aims to develop a rapid method for the detection of ten mycotoxins in oilseeds using amine-functionalized multi-walled carbon nanotubes (NH2-MWCNTs) as adsorbents for dispersive solid-phase extraction (d-SPE) coupled with liquid chromatography-tandem mass spectrometry (LCMS/MS). The structural and morphological characteristics of NH2-MWCNTs were analyzed using X-ray diffraction method, fourier transform infrared spectroscopy, and transmission electron microscopy, confirming increased surface defects after modification. The optimal pretreatment conditions were identified as 2% (V/V) formic acid in acetonitrile-water (80:20) as the extraction solvent and 20 mg NH2-MWCNTs as the adsorbent. The 10 mycotoxins exhibited good linearity within 1-20 μg/kg. The detection and quantitation limits ranged from 0.04 to 0.19 μg/kg and 0.13 to 0.63 μg/kg, respectively. The spiked recovery rates were 70.33%~111.67% and 0.55%~9.60%, respectively. The proposed method is simple, rapid, and environmentally friendly, substantially reducing pre-treatment time and organic solvent consumption. Utilization of NH2-MWCNTs could be an effective approach for determining various mycotoxins in oilseeds.