A hypervirulent persistent genotype (HVPG) Yersinia enterocolitica strain Y2844 was selected as the host for the isolation of a novel lytic Y. enterocolitica bacteriophage, vB_YenP_WW1 (WW1), from sewage samples collected from Guangzhou, China. Genome sequencing revealed that phage WW1 possesses a complete genome of 39,589 bp and shares the highest nucleotide sequence similarity (92.77%) with Escherichia phage P483. Phage WW1 exhibited tolerance to a broad range of temperatures (4~50 ℃) and pH values (5~11). Bactericidal assays revealed that WW1 effectively inhibited Y. enterocolitica growth in LB broth at 28 ℃ for up to 8 h. Following 24 h of phage treatment at 4 ℃, WW1 reduced viable Y. enterocolitica counts on raw pork surfaces from 3.82 log CFU/mL to 2.67 log CFU/mL and decreased viable Y. enterocolitica counts in milk from 5.41 log CFU/mL to below the detection limit. Furthermore, phage WW1 significantly inhibited biofilm formation by Y. enterocolitica on polyethylene and stainless steel surfaces. These findings suggest that phage WW1 possesses strong environmental tolerance and considerable potential as an antimicrobial agent for controlling Y. enterocolitica contamination in food matrices and food processing factories.