Glucoraphanin (RAA) is a glucosinolate widely found in cruciferous plants, the metabolites of which have been established to have anti-cancer effects. However, the purity of RAA extracted from cruciferous plants is low, which limits its effective utilization, and consequently, it is necessary to develop an effective process for RAA purification. Although the main technique currently used for the purification of RAA is chromatographic purification, this procedure has notable drawbacks, including low recovery rates and secondary contamination. As an alternative procedure, membrane purification, which is the most commonly used method for the separation and purification of glycosides, requires only simple equipment and entails simple operation. Moreover, it is less prone to secondary contamination. Accordingly, this separation procedure is considered a potential technique for the purification of RAA. It is speculated that combined chromatographic and membrane purification could facilitate the effective recovery of RAA and enhance the purity of the recovered product, thereby overcoming the problems of low recovery and secondary contamination of RAA associated with the sole use of chromatographic purification. This study focuses mainly on the main methods currently used for the purification of RAA and assesses the feasibility of the combined application of chromatography and membrane purification techniques for RAA purification, thereby providing a methodological reference for the efficient purification of RAA.