In this study, a passage stability-improving method gor Lactobacillus was explored to improve the colonization ability of Lactobacillus in the gastrointestinal tract, which would provide a basis for industrial production. In this research, Lactiplantibacillus plantarun M547, M621 or M748 and Lactiplantibacillus pentosus M750 were used as the research objects to investigate the effect of bile salt addition on the growth of the strains, and the appropriate concentrations of the bile salt culture strains were determined using the growth curve instrument, and the performance of bile salt culture and MRS culture strains in terms of bile salt tolerance, acid production capacity, and acid resistance was compared. After the culture with 0.04% bile salt, the survival rate of the strain with tolerance towards 0.075% bile salt increased by about 11%~18% during passage, and the survival rate of the strain with tolerance towards 0.1% bile salt increased by 20%~34%. Bile salt culture had no obvious effect on the improvement of acid production capacity and acid tolerance and stability, but can improve the bile salt tolerance and passage stability of the strains, which can provide theoretical support for the research of the methods to improve the probiotic properties of probiotics.