To explore the effects of Bifidobacterium animalis CP-9 (CP-9) and Limosilactobacillus reuteri ADR-1 (ADR-1) on weight, fat distribution and blood fatin obese mice. The obese mice fed with high fat diet were randomly divided into model control group (High-fat Diet, HFD), CP-9 group, ADR-1 group. The mice in blank control group (Normal Diet, ND) were fed with normal diet. The body weight and body composition of mice were measured, the distribution of body fat and the pathological changes of liver tissue were observed, the blood biochemical indexes were measured, and the protein expressions of fatty acid synthase (FAS) and peroxisome proliferator-activated receptor γ (PPARγ) in liver tissue were determined. At the end of the intervention, compared with HFD, the body weight (-18.82%, -18.82% vs HFD), abdominal and subcutaneous fat, serum low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and alanine aminotransferase (ALT) of mice in theCP-9 and ADR-1 groups decreased significantly (P<0.05). Liver steatosis and liver injury in CP-9 and ADR-1 groups were significantly improved compared with HFD group, and the protein expression of FAS gene in liver decreased (-53.22%, -53.22% vs HFD), while the protein expression of PPAR γ gene increased (571.43% vs HFD 714.29%). In summary, the intervention of CP-9 andADR-1 can effectively reduce the body weight, abdominal and subcutaneous fat of obese mice, improve hepatic steatosis and liver injury, probably by regulating the protein expression of FAS and PPAR γ genes in liver to reduce TG and LDL-C so as to improve the abnormality of fat metabolism.