To establish a method for detecting sparfloxacin (SPFX) residues in pork, which has the characteristics of high specificity, low background, and no need for sample pretreatment, an indirect competitive enzyme-linked immunosorbent and a specific anti-spafloxacin monoclonal antibody were prepared in this paper. The results showed that the antibody had almost no cross-reactivity with flumequine (11.99%), fleroxacin (11.14%), enrofloxacin (4.60%), and ciprofloxacin (0.31%). This method measured drug residues in pork with SPFX, and the intra-detection coefficient of variation was less than 5.68%, and the inter-detection coefficient of variation was less than 5.51%. The average recovery rates between and within the test were 102%~106% and 101%~104%, respectively. The IC50 of this antibody against SPFX is 31 μg/L, and almost no antibody has four structurally related compounds and other compounds. The results of high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) further confirmed the reliability and accuracy of the rapid detection of SPFX in pork by icELISA. This study shows that the icELISA method to detect SPFX residues in pork is a reliable, simple, and low-cost method.