To study the bactericidal effects of dielectric barrier discharge cold plasma against S. aureus and P. aeruginosa, bacterial suspension (1×108 CFU/mL) was used as the research object to examine its bactericidal effect. Linear, Weibull and Log-linear+Shoulder+Tail models were used to examine the kinetic characteristics of cold plasma sterilization, and root mean square error (RMSE), determination coefficient (R2), accuracy factor (Af) and bias factor (Bf) as the four indices were used to evaluate the fit of the model. The outcome of the sterilization in this experiment showed that under the conditions of O2:N2=65:35, the medium thickness was 2 mm, the treatment voltage was 75 kV and the treatment time was 150 s, the sterilization rates of S. aureus and P. aeruginosa were 90.00% and 98.99%, respectively, just after the treatment (without standing); After the standing for 2 h following the treatment, the sterilization rates for the two strains increased to 90.27% and 99.61%, respectively. The fitting results showed that the Weibull model and the Log-linear+Shoulder+Tail model were more in line with the actual sterilization curves of cold plasma-treated S. aureus and P. aeruginosa than the Linear model, whilst the Log-linear+Shoulder+Tail model has a wider range of applications and can better describe the sterilization process of cold plasma under different standing time periods.