To provide scientific bases for the safe application of Astragalus stem and leaf, acute oral toxicity, genetic toxicity, and 90-day subchronic toxicity tests were performed, following standard procedures for toxicological safety evaluation of food, in mice and rats. The acute oral LD50 of male and female mice and rats was more than 250.00 g/kg BW, indicating that the stem and leaf of Astragalus are non-toxic. Results obtained from all the three genotoxicity tests indicate that the stem and leaf of Astragalus are non-toxic; in addition, mutagenicity was not detected. In the 90-day oral toxicity test, all the rats in each treatment group grew well, and no symptoms of poisoning were observed. There was no significant difference in eye performance, body weight, weight gain, food intake, food utilization efficiency, hematology, blood biochemistry, urine chemistry, organ weight, and organ/body weight ratio and no obvious histopathological changes were found. A dose of 15.00 g/kg BW was found to have no harmful effects on the experimental animals. Results showed the doses of Astragalus stem and leaf used in this study to safe and non-toxic. The findings of this research will provide toxicological data, which can indicate the safe consumption of this product.