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明胶-柠檬酸-硬脂酸复合凝胶的制备及性能
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郭华(1981-),女,博士,研究方向:生物化学、生物基功能材料,E-mail:guohuatyut@126.com 通讯作者:靳利娥(1964-),女,博士,教授,研究方向:食品化学、生物化学,E-mail:lejin2003@163.com

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国家自然科学基金项目(51174144);山西省高等学校科技成果转化培育项目(201930)


Preparation and Properties of Gelatin-citric Acid-stearic Acid Composite Gel
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    摘要:

    针对明胶分子链刚性差,单一组分制备的凝胶机械性能和保水性较差,降解速率快的问题,该研究以明胶为原料,柠檬酸为交联剂,利用疏水性物质硬脂酸对其进行改性,制备明胶-柠檬酸-硬脂酸(Gelatin-Citric Acid-Stearic Acid,GA-CA-SA)复合凝胶。以压缩强度为响应值优化了明胶-硬脂酸复合凝胶的制备工艺条件,利用扫描电子显微镜观察其形貌、红外光谱仪对化学结构进行了表征,并对其进行了细胞毒性评价和姜黄素缓释研究。结果表明:最佳工艺参数为硬脂酸浓度0.013 g/L,柠檬酸浓度0.015 g/mL,温度69 ℃,在此条件下制备的GA-CA-SA复合凝胶压缩强度为99.05 kPa,较明胶(Gelatin,GA)凝胶压缩强度(45.55 kPa)来说,力学强度提高了117%。红外光谱表明,由于柠檬酸的交联作用,改变了明胶的二级结构,使得形成的GA-CA-SA复合凝胶,亲水性的明胶排列在外面,疏水性的硬脂酸包裹在里边,形成了规则的孔隙结构。且无细胞毒性,对姜黄素具有较好的缓慢释放的效果。

    Abstract:

    In order to enhance the mechanical properties of gelatin gel and expand its application value, gelatin-citric acid-stearic acid composite gel was prepared by using gelatin as raw material, citric acid as cross-linking agent and stearic acid as additive. The preparation conditions of composite gel were optimized with compressive strength as the response value. The morphology of the composite was observed with a scanning electron microscope (SEM), the chemical structure was characterized by infrared spectroscopy (FT-IR), and the cytotoxicity and curcumin release were studied. The results showed that the optimized process conditions were as follows: the quality of stearic acid was 0.013 g/mL, the quality of citric acid was 0.015 g/mL, and the temperature was 69 ℃. The mechanical strength of the gelatin-stearic acid composite gel prepared under the optimal conditions was 117% higher than that of the single component gelatin gel (45.55 kPa). The FT-IR spectrum analysis showed that the secondary structure of gelatin was changed due to the cross-linking effect of citric acid, resulting in the formation of GA-CA-SA composite gel. The hydrophilic gelatin was arranged outside and the hydrophobic stearic acid was wrapped in it. Inside, a regular pore structure was formed. It has no cytotoxicity and has a good slow release effect on curcumin.

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郭华,史泽毅,张海霞,邓博,王亚雄,靳利娥*.明胶-柠檬酸-硬脂酸复合凝胶的制备及性能[J].现代食品科技,2021,37(10):171-179.

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  • 收稿日期:2021-02-23
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  • 在线发布日期: 2021-11-01
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