The extent of the improvements in sleep habits of mice treated with Wolfiporia extensa extracts obtained using different solvents were compared using sleep tests, prolonged pentobarbital-induced sleeping time measurements, below-threshold dose pentobarbital-induced hypnosis tests, and pentobarbital-induced sleep latency tests. Changes in the 5-HT, GABA, and DA content in mouse brain tissues were monitored to explore the mechanism by which Wolfiporia extensa extracts obtained using different solvents improve sleep in mice. The results reveal that, the group treated with high-dose aqueous extracts (112.7 mg/kg) show the greatest improvement in their sleep (p<0.05) compared to the blank control. This group is followed by the groups treated with high-dose ethanol extracts (100.8 mg/kg), high-dose polysaccharides (15.6 mg/kg), low-dose aqueous extracts (56.4 mg/kg), and low-dose ethanol extracts (50.4 mg/kg). Analysis also revealed that low-dose polysaccharides (7.8 mg/kg) (p>0.05) treatment induced very little change in the sleep habits of these animals. More specifically, the sleeping time of the mice treated with high-dose aqueous extracts increased remarkably from 1829.67 s to 2979.83 s, and the incidence rate rises from 10% to 60%. In addition, sleep latency was markedly reduced, from 2033.50 s to 1486.25 s, and the sleep improvements associated with these Wolfiporia extensa extracts were shown to coincide with increases in the GABA and 5-HT content and reductions in the DA content in the brain tissues of treated animals. The 5-HT and GABA content reached up to 26.96 pg/mg and 817.40 pg/mg, respectively, in the animals treated with high-dose aqueous extracts while the DA content dropped to 3.41 pg/mg. This data reveals that high-dose aqueous Wolfiporia extensa extracts are the best option for improving sleep in mice, and that this improvement is closely associated with the regulation of GABA, 5-HT, and DA in the brain.