In this study, the adsorption capacity of black fungus towards Cr(VI) was analyzed. The adsorption characteristics of black fungus towards Cr(VI) at room temperature and in the simulated stomach and intestinal environments, as well as the leaching characteristics under different conditions, were examined. The contention of Cr(VI) was determined by HPLC-ICP-MS. The results showed that the adsorption of Cr(VI) on black fungus was greatly influenced by pH. Under the conditions of 25 ℃, pH 1.5, adsorbent concentration of 1.0 g/L, and initial concentration of Cr(VI) 5.0 mg/L, the maximum adsorption amount was 2059.4 mg/kg. Under the same adsorption conditions, the black fungi, either cooked in boiling water for 20 min or soaked in cold water for 120 min, had essentially the same amount of absorbed Cr(VI). The chromatograms of the Cr(VI) contents in the simulated stomach and intestine revealed that due to the adsorption of black fungi, the content of Cr(VI) decreased significantly and eventually disappears completely during the digestion process from the stomach to the intestine. It was verified that after detoxification, the leached Cr(VI) from the Cr(VI)-containing black fungi was analyzed under different conditions, such as leaching agents, temperatures, duration and pH. The results confirmed the detoxifying characteristics of black fungus towards Cr(VI). Black fungus can effectively adsorb Cr(VI) under various conditions, and the ingested black fungus may exert the detoxifying effect towards Cr(VI) through adsorbing Cr(VI). This research provides a technical route for improving the safety of human consumption.