In this work, a strain of lactic acid bacteria HJ-S2 with high effective cholesterol-lowering was isolated from Beak Whale viscera using o-benzaldehyde method, and the probiotic performance of Lactobacillus plantarum HJ-S2 were investigated in vitro. Acid-resistant and cholestate-tolerant were carried out to detect the probiotic performance of the strain, and antibacterial effect on pathogenic bacteria and adhesion ability to human colon cancer cell HT-29 were also studied. Morphology, API50CH and 16S rDNA were used to identify the strain. The results showed thatthe cholesterol-lowering rate of this lactic acid bacteria HJ-S2 was 48.82% in vitro. Under being cultured for 3 h at pH 3 or in solvent containing 0.3% bile salt, the tolerance rate reached 82.73% and 80.62% respectively. The result of antibacterial analysis suggested metabolites of the strain HJ-S2 have certain inhibitory effect on E.coli, S.areaus and Salmonella. Analysis of adhesion to human colon cancer cell HT-29 of 132.52. The strain was identified as Lactobacillus plantarum and labled as HJ-S2 (CGMCC NO: 17720). The Lactobacillus plantarum HJ-S2 has effective high cholesterol-lowering and can provide a reference for further mircro-ecologicol agent development.