The hypoglycemic effect of Roxburghii juice in high, middle and low dose groups on streptozotocin (STZ) -induced type I diabetic mice was studied. The mice were randomly divided into blank group, model group, positive group, high, middle and low dose groups administration by gavage for 4 weeks. Changes in diet and water intake, body weight, fasting blood glucose (FBG), glucose tolerance, glycosylated serum protein (GSP), glycosylated hemoglobin (GHb), serum insulin (INS), liver glycogen and organ index were measured during the study. After continuous gavage for 28 days, the symptoms of polydipsia and polyphagia were significantly improved and weight loss was inhibited. Compared with the model group, FBG in the high, medium and low dose groups of Rosa roxburghii juice decreased by 39.80%, 18.18% and 7.18%, respectively. GHb decreased by 29.85%, 16.04% and 10.52%. GSP decreased by 18.52%, 9.80% and 6.32%. INS increased by 26.13%, 20.60% and 14.21% respectively, the areas under the blood glucose time curve (AUC) were reduced by 44.43%, 22.62% and 14.07%, respectively, liver glycogen levels were increased by 1.39, 1.10 and 1.02 times, respectively, show that thorn pear juice have auxiliary therapy effect on theⅠdiabetes mice.