In this study, the research objects were organic milk powder and convention milk powder. The carbon stable isotope ratio and nitrogen stable isotope ratio of organic milk powder and convention milk powder were studied. Stable isotopes of carbon and nitrogen were analyzed to record the difference between convention milk powder and organic milk powder. The results showed that the overall trend of the mean δ13C value of organic milk powder was more negative than that of conventional milk powder, and the mean δ15N value of organic milk powder was lower than that of conventional milk powder. Although the data range of δ13C and δ15N values of samples were crossed, both δ13C and δ15N values were statistically different for the organic milk powder and conventional milk powder (p<0.05). The different in δ13C and δ15N values between organic milk powder and convention milk powder could be caused by the dairy cow’s diet, fertilizer types and soil characteristics used for milk production. The results of the present study, which is based on preliminary data from a limited sample size, could be highly valuable and helpful for monitoring the authenticity of organic milk powder.