The performance of non-woven fabric in Petrifilm Aerobic Count Plates was evaluated by investigating the types of non-woven fabric, processing technology, theratio of cold water soluble gel and medium and texture characteristics. When the ratio of gel to medium was 3:7, the hardness, fracturability, adhesiveness, cohesiveness, springiness and gumminess were similar with those of 1% agar medium, and the 60 g/m2 of spunlace non-woven fabric was selected as the carrier . The petrifilm plate method based on prepared cold water soluble gel could achieve the same limit of detection (2 CFU/mL) and sensitivity (100%) in colony counting of five testing strains compared with national standard aerobic bacteria plate counting method. The petrifilm plate method based on cold water soluble gel had high accuracy and linearly dependent coefficient (R2=0.996). These two methods have no significant difference (p>0.05). The spunlaced non-woven fabric can be used as a good carrier of petrifilm aerobic count plate.