Almond juice is rich in plant protein. It is loved by a number of consumers and has a broad consumer market. Meanwhile, whether almond juice was adulterated or not was concerned by consumers. In this study, universal primers for 6 species of almonds, peanuts, almonds, peanuts, walnuts, soybeans, sesame and hazelnuts were designed and screened by using plant DNA barcoding technology and PCR technology to detect the peanuts in almond juice. The results showed that both ITS2-2 and trnH-psbA-1 had higher success rate of amplification and sequencing for six species. A hybrid model was designed with peanut genome DNA mixed in almond genome DNA by calculating the extraction rate of peanut genome DNA and almond genome DNA, and theprimer ITS2-2 in 85.80% peanut was almond and primer trnH-psbA-1 in 6.94% peanut was peanut. ITS2-2 and trnH-psbA-1 could be used as a plant DNA barcode combination to identify peanut in almond juice. This study provides a new idea for the detection of such food, which could be used as a reference for relevant researches.