A high-throughput method was established for simultaneous determination of 33 β-agonists, six cephalosporins, six penicillins, three fluoroquinolones, and two glycopeptides in meat products by dispersive solid phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After enzymolysis treatment, the sample was extracted with 0.1% formic acid-acetonitrile (volume fraction), and purified with dispersive solid phase extraction. The resultant sample was analyzed by positive and negative electrospray ionization (ESI+/-) tandem mass spectrometry under multiple reaction monitoring (MRM) mode. The qualitative analysis was performed using retention time and ion abundance ratio, and the quantitative analysis was performed using external standard method. The results showed that the 33 target compounds displayed a good linearity in the concentration range of 1.0~300 μg/L, with correlation coefficients larger than 0.996. The limits of detection (LOD, S/N=3) were 0.5 μg/kg for β-agonists and fluoroquinolones and 3 μg/kg for the others. At spiked levels of 2.0~100 μg/kg, the average recoveries were in the range of 74.9~112%, and the RSDs ranged from 2.2% to 10.8%. The developed method is accurate, sensitive, and suitable for the high-throughput quantitative and qualitative analysis of β-agonist, cephalosporin, penicillin, fluoroquinolone, and glycopeptide multi-residues in meat products.