本平台为互联网非涉密平台,严禁处理、传输国家秘密、工作秘密或敏感信息

酸驼乳发酵乳酸菌菌株的筛选及其发酵特性研究
CSTR:
作者:
作者单位:

作者简介:

熊磊(1992-),男,在读硕士,从事骆驼乳新产品开发研究 通讯作者:张灏(1962-),男,教授,博士生导师,从事食品微生物的资源挖掘与高效利用的教学与科研工作

通讯作者:

中图分类号:

基金项目:

乳业生物技术国家重点实验室开放课题(SKLDB2015-001);国家自然科学基金(3147172)


Screening and Fermentation Characteristics of a Lactic Acid Bacterial Strain in Acid Camel Milk
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    对新疆自然发酵骆驼乳中乳酸菌菌群进行分离鉴定得到五株乳酸菌,其中乳杆菌3株分别为瑞士乳杆菌(Lactobacillus helveticus)、植物乳杆菌(Lactobacillus plantrum)和开菲尔乳杆菌(Lactobacillus kefiri),片球菌2株分别为乳酸片球菌(Pediococcus acidilactici)戊糖片球菌(Pediococcus pentosaceus)。分离鉴定出的五株乳酸菌在培养基中生长良好,其活菌数在培养16 h后能达到109 CFU/mL。将传统酸奶发酵剂接种于骆驼乳中并于42 ℃条件下培养,发现其在骆驼乳中能较好地产酸,其添加量在0.1%时最适。将筛选到的乳酸菌制备成生产发酵剂与传统酸奶发酵剂复配,得到瑞士乳杆菌组的复配效果最佳,具有最优的产酸效果,在经过6 h发酵后pH值降到4.5左右,酸度达到85 oT,且制备的发酵骆驼乳具有最好的感官品质,其感官评分明显高于其他菌株复配进行发酵的骆驼乳产品。因此,可将0.1%传统酸奶发酵剂与筛选到的瑞士乳杆菌制备的生产发酵剂复配应用于酸驼乳发酵。

    Abstract:

    Five strains of lactic acid bacteria (LAB) were isolated and identified in Xinjiang naturally fermented camel milk, including three strains of lactobacilli (Lactobacillus helveticus, Lactobacillus plantrum, and Lactobacillus kefiri) and two strains of Pediococcus acidilactici (Pediococcus acidilactici and Pediococcus pentosaceus). These LAB strains showed good growth in the culture medium, where the viable cell count reached 109 colony-forming units (CFU)/mL after 16 h of cultivation. Traditional yogurt starter culture was inoculated in camel milk and incubated at 42 ℃ and the results showed effective acid production, where optimum acid production was achieved with 0.1% traditional yogurt starter culture. The isolated LAB strains were prepared as fermenting agents combined with traditional yogurt starter culture. The combination using Lactobacillus helveticus exhibited optimal synergistic effect and acid-generating efficiency. After six hours of fermentation, the pH value reached approximately 4.5, with acidity value of 85 oT. Furthermore, the fermented camel milk showed higher sensory quality and a higher sensory evaluation score compared with fermented camel milk samples prepared using combinations of other strains. Therefore, the fermenting agent combination of 0.1% traditional yogurt starter with Lactobacillus helveticus strains can be used for acid camel milk fermentation.

    参考文献
    相似文献
    引证文献
引用本文

熊磊,赵建新,范大明,刘小鸣,闫博文,王丽云,陈卫,张灏.酸驼乳发酵乳酸菌菌株的筛选及其发酵特性研究[J].现代食品科技,2016,32(9):84-89.

复制
分享
相关视频

文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2015-10-10
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2016-09-28
  • 出版日期:
文章二维码