The copper contents of wines from Helan Mountain East region, Hexi Corridor region, Huaizhuo Basin region, and Tonghua region were investigated using inductively coupled plasma-mass spectrometry (ICP-MS). Results showed that the copper content of wines obtained from these four regions was in a good condition overall; the detection rate of excess copper content was 0.2%. Among the four regions, Tonghua region showed the highest copper content, followed by Helan Mountain East region, while Hexi Corridor region and Huaizhuo Basin region showed relatively lower copper contents. The distribution of the copper content in Tonghua wine was polarized. The copper content in the Helan Mountain East region ranged between 50~100 μg/L and 100~300 μg/L, while the Hexi Corridor region and Huaizhuo Basin region showed a more uniform distribution of copper content. Regional climate and terroir characteristics, winery and vineyard management, and winemaking technology exert large effects on the copper content of wines. The copper contents of different wine grape varieties in the same region were similar, but there were certain differences between the wine grape varieties of different regions. Vintage also had a certain influence on copper content, which was mainly related to the climate, especially to precipitation.