Eriocheir sinensis farmed in Yangcheng Lake is a valuable traditional aquatic product and quite welcomed by Chinese consumers due to its outstanding flavor. In this study, a new type of material (MonoTrap) was applied as the adsorbent to extract the volatile compounds in gonad of Eriocheir sinensis. The volatile compounds were then isolated and identified by GC-MS. The optimal extraction conditions were using seven MonoTrap RCC18 at 100 ℃ for 50 minutes. 55 volatiles that could be divided into seven classes were finally detected under the forementioned conditions. Odor activity values (OAV) of all 55 volatiles were calculated according to their odor thresholds and 22 aroma-active compounds (AACs) were further selected. Among the AACs, 2,4-decadienal, trimethylamine and decanal mainly contributed to integral aroma profile of the gonad sample. Monolithic Material Sorptive Extraction (MMSE) is considered as an excellent method for food flavor analysis because of its high efficiency of volatile compounds absorption.