In the present work, the heterotrophic growth of Chlorella pyrenoidosa and Scenedesmus dimorphus NIES-119 were analyzed to investigate the tolerant concentrations of sucrose and the cell tolerance capacity to high concentration wastewater from yeast fermentation. The results showed that Chlorella pyrenoidosa and Scenedesmus dimorphus NIES-119 had high specific growth rates (0.90 d-1 and 0.63 d-1, respectively) in medium containing high concentration of sucrose up to 70 g/L, and the maximal biomass concentrations reached to 3.37 g/L and 2.84 g/L, respectively. After adding molasses in the wastewater medium, the chemical oxygen demand (COD) of 104~ 105 mg/L was observed with high concentration of total nitrogen and phosphorus. The maximal specific growth rates and highest biomass concentrations were 0.43 d-1 and 1.95 g/L, respectively, for Chlorella pyrenoidosa, and were 0.42 d-1 and 1.70 g/L, respectively, for Scenedesmus dimorphus NIES-119, which were significantly higher than the maximum in the molasses-free wastewater medium. Furthermore, the highest removal rates of COD, total nitrogen and total phosphorus were 34.56%, 20.63% and 20.00%, respectively, by Chlorella pyrenoidosa and 21.46%, 16.11% and 31.25%, respectively, by Scenedesmus dimorphus NIES-119. These resultsdemonstrated the two kinds of microalgae had feasibility and great potential for wastewater cleaning.