To know the performance of the kit, indirect competitive enzyme-linked immunosorbent assay(ELISA) was used to detect the residues of zearalenone in the 3 kinds of corn oil. The results showed that the linear equation was: y=-2.2x+0.66, r=0.9974, IC50=0.29 μg/kg. Quantitation of zearalenone was linear from 0.05 μg/kg to 4.05 μg/kg. The sensitivity was 0.05 μg/kg. The recovery rates in the corn oil were more than 85%. The sensitivity, repeatability and specificity of the method were satisfactory and could meet the need of detecting the residues of zenralenone in corn oil.