The content of aflatoxin B1 in tartary buckwheat products was detected with ELISA kit, and the prevention and control measures of aflatoxin B1 were discussed. The aflatoxin B1 content of tartary buckwheat nutrition power was the least in the 4 kinds of produces analyzed, followed by tartary buckwheat flast dissolving tablets and tartary buckwheat vinegar. The aflatoxin B1 content of tartary buckwheat tea was the highest. The detection sensitivity was 0.1 μg/kg and the average spike recovery rate was 84.70~84.95%. The relative standard deviation was less than 5%. The results showed that the ELISA was accurate with good stability and reproducibility, so it can be applied to detect aflatoxin B1 in tartary buckwheat products widely.