Quantitative detection of the clenbuterol in pig urine was investigated with a clenbuterol kit based on immunofluorescence chromatography and a fluorescence quantitative detector. The detection limit was up to 0.26 μg/L and the cross-reaction with the chemical drugs of similar structure was less than 0.20%. And the experiment confirmed that the kit had 1-year shelf life. The total detection process just takes about 20 minutes.