In order to detect the Vibrio parahaemolyticus in the import and export foods more accurately and quickly, the study designed the loop-mediated isothermal amplification (LAMP) primer sets targeted the tlh gene of V. parahaemolyticus, and detected the LAMP results real-time by the ESEQuant tube scanner. The results showed that the reaction characterized with high efficiency, high specificity and high sensitivity (1~10 CFU/reaction, about 100 times than the real-time PCR). One hundred and twenty-six out of 126 V. parahaemolyticus strains were detedted as Vibrio parahaemolyticus by the LAMP assay with the ESEQuant tube scanner, while 121 strains were detected as V. parahaemolyticus by the real-time PCR. The ESEQuant tube scanner is comparable to the real-time PCR machine in the sense that both are capable of detecting samples in real-time resulting and both are capable of conducting melting curve analysis, which means the ESEQuant tube scanner system can both served as research application in basic laboratory or offering pre-treatment diagnosis in primary health care settings in developing areas.