Through acute toxicity test, genetic toxicity test, traditional teratogenic test and 30-day feeding test, the wolfberry beverage was confirmed as safe and non-toxic. Preliminary evaluation of its alleviate eyestrain effect on human showed that the visual fatigue symptoms of 66.67% of the subjects were obviously alleviated. The bright durability of their eyes was improved by an average of 17.15 ±8.37% and the distant vision was improved by 0.14 ± 0.07%. Statistical analysis showed a significant difference in the results.