The desalting technology of antioxidant peptides from tilapia skin collagen by DA201-C macroporous adsorption resin was investigated. The results showed that the static absorption rate and absorbing capacity were 82.56% and 54mg/g, respectively. The best loading sample concentration, sampling flow rate and eluent were 60 mg/mL, 1 mL/min and 75% alcohol, respectively. Under these conditions, the desalination rate was 97.13% and recovery of peptides was 81.16%. The antioxidant activity was increased to 39.58% at the concentration of 0.245 mg/mL. In conclusion, it was available and effective to desalt the antioxidant peptides from tilapia skin collagen by using DA201-C macroporous absorption resin.