The tensile strength, water vapor permeability, water absorption and the contact angle of zein film were investigated, in order to study the influence of isopropanol concentrations on the properties of zein film. Experimental results showed that increase in the isopropanol content of solvent, the tensile strength of zein film increased until it reached a maximum about 57.7 MPa at 90% isopropanol content. As the isopropanol content of solvent increased from 70% to 95%, both WVP and water absorption of the zein film decreased and the minimum were about 0.075?10-8g?m/(m2?h?Pa) and 37.2%, respectively, at 90% isopropanol content. The contact angle experiment indicated that contact angle of zein film produced from 90% isopropanol solution was 67°and showed good waterproof properties. Measurement of AFM showed that zein dissolved in isopropyl alcohol solution formed orderly agglomerations and agglomerations condensed each other, resulting in a network structure. DSC results showed that denaturation temperature of zein film was higher than zein degeneration temperature.