This paper mainly reported the differences of physical and chemical composition of Lycium barbarum L. dried fruit in eight areas ( Nanliang, Huinong, Guyuan, Zhongning, Inner Mongolia, Gansu, Qinghai and Xinjiang), thus to compare the quality differences of Lycium barbarum L. in different producing areas. Seven kinds of physical and chemical composition and three kinds of active ingredient were determined. Also variance, significant analysis, weighted scoring and clustering analysis of the results were made. The results showed that the comprehensive weighted score and the order of comprehensive quality of Lycium barbarum L. in different areas as follows, Zhongning 7.3>Nanliang 5.1>Xinjiang 4.8>Huinong 4.5>Gansu 3.8>Qinghai 3.7>Inner Mongolia 3.6>Guyuan 3.2. The qualities of Lycium barbarum L. in NanLiang and HuiNong are very close to Zhongning’s. It was determined that the center of superior Lycium barbarum L. was in Ningxia, but the place has transferred to Nanliang and Huinong from Zhongning.