The purification technique of camellia saponin by mixed microbial fermentation was studied via using oil Camellia seed wastewater as raw materials. The optimal results were achieved by Candida rugosa and Aspergillus oryzae. The optimal fermented condition were the total inoculation quantity 8%, the ratio of inoculation 2:3 and the fermentation time 4 days. By liquid fermentation tests under the above mentioned conditions, protein content was reduced by 60.99% and polysaccharide content was lowered by 54.02%. Purity of camellia saponin improved from 36.45% to 58.74% and the color of camellia saponin products was changed from dark brown to pale brown.