The response surface methodology was used to optimize the extraction of Fagopyrum of Miqiao 1. Based on one-factor-at-a-time experiments, the extraction of time, temperature, liquid-to-solid ratio and methanol concentration were used to carry out a 4-variable, 3-level central composite design, and established quadratic regression model. The best extraction time，temperature, solid-liquid ratio and methanol content were 93 minute, 77 ℃, 1:32 and 91 % under which the predicted yield of rutin with the optimized procedure was 17.92 mg/ and the practical yield of rutin was 18.20 mg/g.