Production of monolauroyl fructose catalyzed by immobilized Lecitase? Ultra in anhydrous 2-methyl-2-butanol was investigated in this study. The process parameters was optimized as follows: reaction medium 4 mL, biocatalyst 25 g/L, glucose 250 mmol/L, lauric acid 1000 mmol/L, molecular sieves load 100 g/L, temperature 43 ℃. The maximum monolauroyl fructose conversion could reach 69.45% after 48 h.