The extraction process of α-glucosidase inhibitor from Glycyrrhizins uralensis Fisch were investigated in this article. With single factor experiment and the orthogonal experiment designed, the best extracting reagent was detemined as water and the best extraction process were confirmed as follows: extraction temperature 30 ℃, extraction time 1 hour and ratio of solid to liquid 1:10, under which 48.94% of α-glucosidase inhibitor was abtained. The effects of the extraction parameters on α-glucosidase yield were in order of extracting time >temperature>ratio of solid to liquid.