Using spectrophotometry, Oxford cup and two-fold dilution methods were adopted to study the antioxidant capacity, antimicrobial capability and anti-inflammatory capability of total flavonoids from Carica Papaya L. Leaves. The results showed that total flavonoids from Carica Papaya L. Leaves had great capability on antioxidant, antimicrobial and anti-inflammatory. It had stronger reducing capability than VC and BHT at the same concentration. And the MIC of the extracts on E. coli, S. aureus and Bacillus subtilis were 8, 4 and 4 mg/mL, respectively.And the MBC on E. coli, S. aureus and Bacillus subtilis was 8, 4 and 4 mg/mL, respectively. The IC50 of anti-inflammatory capability was 1.93mg/mL.