The effects of H22 cells vaccine treated with carotene degradants as immunoadjuvant on the mice were studied in this paper. By immunising the mice with the coculture of H22 cells and carotene degradants, setting the model and the control, it was found that the carotene degradants had no toxic effects on the mice and the cancer inhibition rate of immue mice was 93%. Carbon particle clearance and the phagocytsis index of macrophages were 0.39±0.002 and 5.42±0.19, respectively. Cytotoxicity of NK cells was 0.93. All of the parameters had significant difference compared with those of the model. The research proved that H22 cells vaccine treated with carotene degradants can effectively protect immune organs.