The extraction process parameters of salt-soluble protein were studied with the muscle of Penaeus Vannamei as raw materials and the salt- soluble protein yield as an indicator in this experiment. The optimized extraction conditions were determined as follows:NaCl 0.6 mol/L, extraction time 25 h, solid-liquid ratio 1:4, and pH 7. Under these conditions, the salt-soluble protein content was 105.73 mg/g and yield was 83.34%.