D-Glucono delta-lactone was produced from D-Gluconic acid using molecular sieve MCM-41 as catalyst. Effects of various factors on D-Glucono delta-lactone yield, including reaction temperature, reaction time, catalyst dosage and recycling index of molecular sieve MCM-41, were discussed. Experimental results showed that molecular sieve MCM-41 was a better catalyst for preparation of D-Glucono delta-lactone. The optimum reaction condition was obtained as follow: catalyst dosage 30%, reaction temperature 80 ℃, and reaction time 2 h, under which D-Glucono delta-lactone yield was as high as 90.39%.