Enzyme plays an important role in tobacco curing, aging and storing management. How to use the enzyme to improve smoking quality has become a hot topic in tobacco industry in recent years. Enzyme can degrade the excessive starch, protein, cell wall substances and the pectin in tobacco leaves, enhance the fragrances and improve the quality of the tobacco. Besides, the applications of enzymes on the tobacco additives, tobacco flavor and reconstituted tobacco production are reviewed and researches on the combination of enzyme and microorganism used in tobacco industry are also introduced here.