The General nutrients of squid viscera were analyzed and the extraction of glycoprotein from squid viscera by NaCl solution was investigated. The immunoloregulation effect of the glycoprotein was also researched. Results showed that squid viscera contained high contents of proteins. The optimum extraction conditions were as follows: ratio of solid to liquid of 1:6, extraction time of 60 min, NaCl content of 3% and temperature of 80℃. Our experiments also confirmed the extracted glycoprotein possessed immunomodulation activity.