The enzymatic method for the extraction of capsaicin by cellulose (16568 U/g) was developed. The results showed that the best enzymatic hydrolysis conditions for extracting capsaicin were as follows: pH value of enzyme solution of 5.2, the enzyme dosage of 7 mg/g, the reaction time of 3 h, and the reaction temperature of 40 ℃. Compared with the traditional extraction methods, the extraction yield of capsaicin with the new method increased by 16%. Besides, this extraction method was simple, fast and with relative low temperature.