The contents of estradiol, progesterone and testosterone in muscle of Lutjanus argentimaculatus in Zhanjiang sea area were determined by RIA. It was found that the contents of the three steroid hormones in muscle of cultured Lutjanus argentimaculatus were 4.50, 1.57, and 5.00 times as those in wild Lutjanus argentimaculatus, respectively. The residue levels of steroid hormones in wild Lutjanus argentimaculatus were determined to be very low and were possibly correlated with environmental hormones. It suggested that RIA could be regard as an efficient method for determining the hormone residues in aquatic products.