Yeast-like strain XYE-7 for xylanase production was isolated from soil. The best carbon sources and nitrogen sources for the fermentation were as follows: 0.5% xylan, 0.5% (NH4)SO4, 0.2% barmene and 0.1% KH2PO4. The optimal initial pH value, culture temperature and fermentation time were 2.0, 35 ℃ and 80 h, respectively.